Characterization of the binding of cAMP and cGMP to the CRP*598 mutant of the E. coli cAMP receptor protein.

doi:10.1093/nar/18.17.5127

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Article

Characterization of the binding of cAMP and cGMP to the CRP*598 mutant of the E. coli cAMP receptor protein.

Nucleic acids research - 11 Sept 1990

Ren Y L, Garges S, Adhya S, Krakow J S

Abstract excerpt

Wild type cAMP receptor protein (CRP) activates in vitro lac transcription only in the presence of cAMP. In contrast the mutant CRP*598 (Arg-142 to His, Ala-144 to Thr) can activate lac transcription in the absence of cyclic nucleotide or at concentrations of cAMP below that required by CRP. To f...

Topics

Adenosine
Ammonium Sulfate
Binding, Competitive
Chemical Precipitation
Cyclic AMP
Cyclic GMP
Escherichia coli
Mutation
Receptors, Cyclic AMP
Transcription, Genetic

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