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Article

Crystallization and preliminary X-ray diffraction analysis of D53H mutant Escherichia coli cAMP receptor protein.

Acta crystallographica. Section F, Structural biology and crystallization communications - 1 Dec 2013

Huang Jing, Wu Tong, Guo Zheng, Lou Tiantian, Yu Shaoning, Gong Weimin, Ji Chaoneng

Abstract excerpt

The Escherichia coli cyclic AMP receptor protein (CRP) is a prokaryotic global transcription activator protein that controls the expression of many different genes. Wild-type CRP can bind to special DNA sequences in the presence of cAMP. The substitution of Asp53 by His results in the CRP* phenotype, which does not require exogenous cAMP. In the present study, the D53H CRP mutant was overexpressed, purified and...

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Source

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Identifiers

PMID: 24316848
DOI: 10.1107/S174430911303145X
PMCID: PMC3855738

Genes

CRP

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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