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Article

Real-time monitoring of RAG-catalyzed DNA cleavage unveils dynamic changes in coding end association with the coding end complex.

Nucleic acids research - 1 Jul 2012

Wang Guannan, Dhar Kajari, Swanson Patrick C, Levitus Marcia, Chang Yung

Abstract excerpt

During V(D)J recombination, the RAG1/2 recombinase is thought to play an active role in transferring newly excised recombination ends from the RAG post-cleavage complex (PCC) to the non-homologous end joining (NHEJ) machinery to promote appropriate antigen receptor gene assembly. However, this transfer mechanism is poorly understood, partly because of the technical difficulty in revealing weak association of...

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Source

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Identifiers

PMID: 22434887
DOI: 10.1093/nar/gks255
PMCID: PMC3401440

Genes

RAG2
RAG1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Real-time monitoring of RAG-catalyzed DNA cleavage unveils dynamic changes in coding end association with the coding end complex.PMID 22434887Read ↗

Full-length RAG1 promotes contact with coding and intersignal sequences in RAG protein complexes bound to recombination signals paired in cis.Semantic proximity Read ↗

The DDE motif in RAG-1 is contributed in trans to a single active site that catalyzes the nicking and transesterification steps of V(D)J recombination.Semantic proximity Read ↗

Evidence of a critical architectural function for the RAG proteins in end processing, protection, and joining in V(D)J recombination.Semantic proximity Read ↗

Single-molecule analysis of RAG-mediated V(D)J DNA cleavage.Semantic proximity Read ↗

RAG proteins shepherd double-strand breaks to a specific pathway, suppressing error-prone repair, but RAG nicking initiates homologous recombination.Semantic proximity Read ↗

Understanding how the V(D)J recombinase catalyzes transesterification: distinctions between DNA cleavage and transposition.Semantic proximity Read ↗

Hairpin coding end opening is mediated by RAG1 and RAG2 proteins.Semantic proximity Read ↗

Autoinhibition of DNA cleavage mediated by RAG1 and RAG2 is overcome by an epigenetic signal in V(D)J recombination.Semantic proximity Read ↗

Role of recombination activating genes in the generation of antigen receptor diversity and beyond.Semantic proximity Read ↗

Detection of RAG protein-V(D)J recombination signal interactions near the site of DNA cleavage by UV cross-linking.Semantic proximity Read ↗

The end-joining reaction in V(D)J recombination.Semantic proximity Read ↗

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