Article
Understanding how the V(D)J recombinase catalyzes transesterification: distinctions between DNA cleavage and transposition.
Nucleic acids research - 1 May 2008
Lu Catherine P, Posey Jennifer E, Roth David B
Abstract excerpt
The Rag1 and Rag2 proteins initiate V(D)J recombination by introducing site-specific DNA double-strand breaks. Cleavage occurs by nicking one DNA strand, followed by a one-step transesterification reaction that forms a DNA hairpin structure. A similar reaction allows Rag transposition, in which the 3'-OH groups produced by Rag cleavage are joined to target DNA. The Rag1 active site DDE triad clearly plays a...
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