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Article

Autoinhibition of DNA cleavage mediated by RAG1 and RAG2 is overcome by an epigenetic signal in V(D)J recombination.

Proceedings of the National Academy of Sciences of the United States of America - 28 Dec 2010

Grundy Gabrielle J, Yang Wei, Gellert Martin

Abstract excerpt

Gene assembly of the variable domain of antigen receptors is initiated by DNA cleavage by the RAG1-RAG2 protein complex at sites flanking V, D, and J gene segments. Double-strand breaks are produced via a single-strand nick that is converted to a hairpin end on coding DNA and a blunt end on the neighboring recombination signal sequence. We demonstrate that the C-terminal regions of purified murine RAG1 (aa...

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Share to DNA Cleavage
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 21149691
DOI: 10.1073/pnas.1014958107
PMCID: PMC3012499

Genes

RAG1
RAG2

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

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Autoinhibition of DNA cleavage mediated by RAG1 and RAG2 is overcome by an epigenetic signal in V(D)J recombination.PMID 21149691Read ↗

Understanding how the V(D)J recombinase catalyzes transesterification: distinctions between DNA cleavage and transposition.Semantic proximity Read ↗

Role of recombination activating genes in the generation of antigen receptor diversity and beyond.Semantic proximity Read ↗

Crystal structure of the V(D)J recombinase RAG1-RAG2.Semantic proximity Read ↗

Evidence of a critical architectural function for the RAG proteins in end processing, protection, and joining in V(D)J recombination.Semantic proximity Read ↗

The DDE motif in RAG-1 is contributed in trans to a single active site that catalyzes the nicking and transesterification steps of V(D)J recombination.Semantic proximity Read ↗

RAG2 abolishes RAG1 aggregation to facilitate V(D)J recombinationSemantic proximity Read ↗

The RAG1/RAG2 complex constitutes a 3' flap endonuclease: implications for junctional diversity in V(D)J and transpositional recombination.Semantic proximity Read ↗

Hairpin coding end opening is mediated by RAG1 and RAG2 proteins.Semantic proximity Read ↗

RAG proteins shepherd double-strand breaks to a specific pathway, suppressing error-prone repair, but RAG nicking initiates homologous recombination.Semantic proximity Read ↗

Mapping and Quantitation of the Interaction between the Recombination Activating Gene Proteins RAG1 and RAG2.Semantic proximity Read ↗

Requirements for DNA hairpin formation by RAG1/2.Semantic proximity Read ↗

Detection of RAG protein-V(D)J recombination signal interactions near the site of DNA cleavage by UV cross-linking.Semantic proximity Read ↗

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