Article
Hairpin coding end opening is mediated by RAG1 and RAG2 proteins.
Molecular cell - 1 Dec 1998
Besmer E, Mansilla-Soto J, Cassard S, Sawchuk D J, Brown G, Sadofsky M, Lewis S M, Nussenzweig M C, Cortes P
Abstract excerpt
Despite the importance of hairpin opening in antigen receptor gene assembly, the molecular machinery that mediates this reaction has not been defined. Here, we show that RAG1 plus RAG2 can open DNA hairpins. Hairpin opening by RAGs is not sequence specific, but in Mg2+, hairpin opening occurs onl...
Topics
- Alcohols
- Base Pairing
- Base Sequence
- DNA
- DNA-Binding Proteins
- Homeodomain Proteins
- Hydrolysis
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Oligonucleotides