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Article

Single-molecule analysis of RAG-mediated V(D)J DNA cleavage.

Proceedings of the National Academy of Sciences of the United States of America - 7 Apr 2015

Lovely Geoffrey A, Brewster Robert C, Schatz David G, Baltimore David, Phillips Rob

Abstract excerpt

The recombination-activating gene products, RAG1 and RAG2, initiate V(D)J recombination during lymphocyte development by cleaving DNA adjacent to conserved recombination signal sequences (RSSs). The reaction involves DNA binding, synapsis, and cleavage at two RSSs located on the same DNA molecule and results in the assembly of antigen receptor genes. We have developed single-molecule assays to examine RSS binding...

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Identifiers

PMID: 25831509
DOI: 10.1073/pnas.1503477112
PMCID: PMC4394307

Genes

RAG1
HMGB1
RAG2

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Single-molecule analysis of RAG-mediated V(D)J DNA cleavage.PMID 25831509Read ↗

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Real-time monitoring of RAG-catalyzed DNA cleavage unveils dynamic changes in coding end association with the coding end complex.Semantic proximity Read ↗

Detection of RAG protein-V(D)J recombination signal interactions near the site of DNA cleavage by UV cross-linking.Semantic proximity Read ↗

Role of recombination activating genes in the generation of antigen receptor diversity and beyond.Semantic proximity Read ↗

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