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Article

Negative Supercoiling Creates Single-Stranded Patches of DNA That Are Substrates for AID–Mediated Mutagenesis

9 Feb 2012

Abstract excerpt

Antibody diversification necessitates targeted mutation of regions within the immunoglobulin locus by activation-induced cytidine deaminase (AID). While AID is known to act on single-stranded DNA (ssDNA), the source, structure, and distribution of these substrates in vivo remain unclear. Using the technique of in situ bisulfite treatment, we characterized these substrates-which we found to be unique to actively...

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PMID: 22346767
DOI: 10.1371/journal.pgen.1002518

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Negative Supercoiling Creates Single-Stranded Patches of DNA That Are Substrates for AID–Mediated MutagenesisPMID 22346767Read ↗

Targeting of the activation-induced cytosine deaminase is strongly influenced by the sequence and structure of the targeted DNA.Semantic proximity Read ↗

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