Article
The functional properties of full length and mutant chicken gizzard smooth muscle caldesmon expressed in Escherichia coli.
FEBS letters - 17 Sept 1990
Redwood C S, Marston S B, Bryan J, Cross R A, Kendrick-Jones J
Abstract excerpt
Wild type chicken gizzard caldesmon (756 amino acids) was expressed in a T7 RNA polymerase-based bacterial expression system at a yield of 1 mg pure caldesmon per litre bacterial culture. A mutant composed of amino acids 1-578 was also constructed and expressed. The wild type and mutant caldesmon were purified and compared with native chicken gizzard caldesmon. Native and wild type expressed caldesmon were...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.