The functional properties of full length and mutant chicken gizzard smooth muscle caldesmon expressed in Escherichia coli.

doi:10.1016/0014-5793(90)81233-e

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Article

The functional properties of full length and mutant chicken gizzard smooth muscle caldesmon expressed in Escherichia coli.

FEBS letters - 17 Sept 1990

Redwood C S, Marston S B, Bryan J, Cross R A, Kendrick-Jones J

Abstract excerpt

Wild type chicken gizzard caldesmon (756 amino acids) was expressed in a T7 RNA polymerase-based bacterial expression system at a yield of 1 mg pure caldesmon per litre bacterial culture. A mutant composed of amino acids 1-578 was also constructed and expressed. The wild type and mutant caldesmon...

Topics

Animals
Calmodulin-Binding Proteins
Chickens
Escherichia coli
Gizzard, Avian
Mutation
Recombinant Proteins
Structure-Activity Relationship

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