Article
Trifluoperazine binding to mutant calmodulins.
Biochemistry - 22 Jan 1991
Massom L R, Lukas T J, Persechini A, Kretsinger R H, Watterson D M, Jarrett H W
Abstract excerpt
Trifluoperazine (TFP) binding by 14 calmodulins, including 12 produced by site-directed mutagenesis, was determined. While vertebrate calmodulin binds 4.2 +/- 0.2 equiv of TFP, Escherichia coli expressed but unmutated calmodulins bind about 5.0 +/- 0.5 equiv of TFP. The cause for this difference...
Topics
- Amino Acid Sequence
- Animals
- Binding Sites
- Brain Chemistry
- Calmodulin
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Swine
- Trifluoperazine