Article
Alteration in folding efficiency and conformation of recombinant human tumor necrosis factor-alpha by replacing cysteines 69 and 101 with aspartic acid 69 and arginine 101.
Protein engineering - 1 Aug 1990
Arakawa T, Visger J V, McGinley M, Rohde M F, Fox G M, Narhi L O
Abstract excerpt
An analog of human tumor necrosis factor-alpha (TNF-alpha) was created involving the replacement of Cys69 with Asp and Cys101 with Arg. The solution structure and behavior of this analog were compared with the native protein. The analog exhibited a greatly decreased folding efficiency following d...
Topics
- Amino Acid Sequence
- Chemical Phenomena
- Chemistry, Physical
- Circular Dichroism
- Humans
- Mutation
- Protein Conformation
- Protein Engineering
- Recombinant Proteins
- Spectrometry, Fluorescence
- Tumor Necrosis Factor-alpha