Production and characterization of receptor-specific TNF muteins.

doi:10.1385/1-59259-771-8:033

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Article

Production and characterization of receptor-specific TNF muteins.

Methods in molecular medicine - 1 Jan 2004

Ameloot Paul, Brouckaert Peter

Abstract excerpt

Tumor necrosis factor (TNF) is a pleiotropic cytokine with a wide range of biological activities including cytotoxicity, immune-cell proliferation, and mediation of inflammatory responses. Mutational analysis of mature TNF has been facilitated by the high expression levels that were obtained in Escherichia coli cells. Furthermore, the fact that mature TNF does not form inclusion bodies, but remains soluble in...

Topics

Animals
Biological Assay
Cell Division
Cell Line
Escherichia coli
Genetic Techniques
Humans
Mice
Mutagenesis
Mutation
Oligonucleotides
Plasmids
Protein Binding
Radioligand Assay
Tumor Necrosis Factor-alpha

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Open in PubMed

Identifiers

PMID: 15064431
DOI: 10.1385/1-59259-771-8:033

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