Article
A novel tumor necrosis factor-alpha mutant with significantly enhanced cytotoxicity and receptor binding affinity.
Biochemistry and molecular biology international - 1 May 1998
Shin N K, Lee I, Chang S G, Shin H C
Abstract excerpt
A novel tumor necrosis factor-alpha mutant (mutant M3), in which Ser and Tyr at positions 52 and 56 were substituted by Ile and Phe, respectively, along with deletion of 7 N-terminal amino acids, was prepared and its biological activities were investigated. The mutant exhibited a 14- to 24-fold i...
Topics
- Animals
- Antigens, CD
- Drug Screening Assays, Antitumor
- Humans
- Mice
- Mutation
- Neoplasms
- Neoplasms, Experimental
- Protein Conformation
- Receptors, Tumor Necrosis Factor
- Receptors, Tumor Necrosis Factor, Type I
- Receptors, Tumor Necrosis Factor, Type II
- Structure-Activity Relationship
- Tumor Cells, Cultured
- Tumor Necrosis Factor-alpha