Article
Rapid nonlysosomal degradation of assembled HLA class II glycoproteins incorporating a mutant DR alpha-chain.
Journal of immunology (Baltimore, Md. : 1950) - 15 Oct 1990
Koppelman B, Cresswell P
Abstract excerpt
Gamma irradiation followed by antibody and complement selection was used to isolate a human B-lymphoblastoid cell line that no longer expresses HLA-DR molecules on its cell surface. Cell surface expression in the mutant (HMy2.DRN) was restored by transfecting a wildtype DRA but not a DRB cDNA, suggesting that a structural mutation in the DRA mRNA or protein was responsible for the lack of cell surface expression....
Topics
- Amino Acid Sequence
- B-Lymphocytes
- Base Sequence
- Blotting, Northern
- Cell Compartmentation
- Cell Line
- Cloning, Molecular
- DNA
- Electrophoresis, Gel, Two-Dimensional
- Gene Expression
- HLA-DR Antigens