Article
Construction of Aspergillus niger lipase mutants with oil-water interface independence.
Enzyme and microbial technology - 8 Feb 2011
Shu Zhengyu, Wu Jiguang, Xue Longyin, Lin Ruifeng, Jiang Yongmei, Tang Lianghua, Li Xin, Huang Jianzhong
Abstract excerpt
Based on previous bioinformational analytical results [Shu ZY, et al. Biotechnol Prog 2009;25:409-16], four A. niger lipase (ANL) mutants, ANL-Ser84Gly, ANL-Asp99Pro, ANL-Lys108Glu and ANL-EαH (obtained by replacing the lid domain of ANL with the corresponding domain from A. niger feruloyl esterase), were constructed to screen out ANL mutants with oil-water interface independence. ANL-S84G displayed a pronounced...
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