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Article

Activity enhancement of Candida antarctica lipase B by flexibility modulation in helix region surrounding the active site.

Applied biochemistry and biotechnology - 1 Jun 2013

Hong So Yeon, Yoo Young Je

Abstract excerpt

The activity of Candida antarctica lipase B was improved by mutation of the area surrounding the active site. We changed the edges of four helices surrounding the active site to flexible amino acids. Two mutants, V139E and I255E, obtained as a result of Pichia pastoris expression, showed enhanced...

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Identifiers

PMID: 23625607
DOI: 10.1007/s12010-013-0237-8

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-23625607-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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