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Article

Removing the Active-Site Flap in Lipase A from Candida antarctica Produces a Functional Enzyme without Interfacial Activation.

Chembiochem : a European journal of chemical biology - 1 Jan 2016

Wikmark Ylva, Engelmark Cassimjee Karim, Lihammar Richard, Bäckvall Jan-E

Abstract excerpt

A mobile region is proposed to be a flap that covers the active site of Candida antarctica lipase A. Removal of the mobile region retains the functional properties of the enzyme. Interestingly interfacial activation, required for the wild-type enzyme, was not observed for the truncated variant, although stability, activity, and stereoselectivity were very similar for the wild-type and variant enzymes. The variant...

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PMID: 26543016
DOI: 10.1002/cbic.201500471

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