Article
Site-directed mutagenesis studies of the aromatic residues at the active site of a lipase from Malassezia globosa.
Biochimie - 1 Jul 2014
Gao Chongliang, Lan Dongming, Liu Lu, Zhang Houjin, Yang Bo, Wang Yonghua
Abstract excerpt
The lipase from Malassezia globosa (SMG1) has specific activity on mono- and diacylglycerol but not on triacylglycerol. The structural analysis of SMG1 structure shows that two bulky aromatic residues, W116 and W229, lie at the entrance of the active site. To study the functions of these two residues in the substrate recognition and the catalytic reaction, they were mutated to a series of amino acids....
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