Deletion of sites for initiation of DNA synthesis in the origin of broad host-range plasmid R1162.

doi:10.1016/0022-2836(90)90286-u

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Article

Deletion of sites for initiation of DNA synthesis in the origin of broad host-range plasmid R1162.

Journal of molecular biology - 5 Aug 1990

Zhou H S, Meyer R J

Abstract excerpt

The origin of replication of the broad host-range plasmid R1162 contains two, oppositely facing initiation sites for DNA synthesis. Either of these sites can be deleted from an R1162 plasmid derivative. However, the resulting plasmids are unstable, maintained at a lower copy-number in the cell, a...

Topics

Base Sequence
DNA Replication
DNA, Bacterial
DNA, Recombinant
DNA, Superhelical
Escherichia coli
Molecular Sequence Data
Mutation
R Factors
Restriction Mapping
Transformation, Bacterial

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