Transcription-induced deletions in plasmid vectors: M13 DNA replication as a source of instability.

doi:10.1007/BF02173004

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Article

Transcription-induced deletions in plasmid vectors: M13 DNA replication as a source of instability.

Molecular & general genetics : MGG - 25 Sept 1996

Vilette D, Ehrlich S D, Michel B

Abstract excerpt

We have previously shown that concurrent progression of pBR322 replication and pTac-directed transcription in opposite orientations induces illegitimate recombination events. We tested here the effects of M13 rolling circle replication on the incidence of plasmid deletions. The progression of the...

Topics

Adenosine Triphosphatases
Bacteriophage M13
DNA Helicases
DNA Replication
Escherichia coli
Lac Operon
Mutation
Nuclear Proteins
Plasmids
Replication Origin
Sequence Deletion
Transcription, Genetic
Transformation, Genetic

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