Article
Fidelity of mammalian DNA replication and replicative DNA polymerases.
Biochemistry - 24 Dec 1991
Thomas D C, Roberts J D, Sabatino R D, Myers T W, Tan C K, Downey K M, So A G, Bambara R A, Kunkel T A
Abstract excerpt
Current models suggest that two or more DNA polymerases may be required for high-fidelity semiconservative DNA replication in eukaryotic cells. In the present study, we directly compare the fidelity of SV40 origin-dependent DNA replication in human cell extracts to the fidelity of mammalian DNA polymerases alpha, delta, and epsilon using lacZ alpha of M13mp2 as a reporter gene. Their fidelity, in decreasing...
Topics
- Base Sequence
- DNA
- DNA Polymerase II
- DNA Polymerase III
- DNA Replication
- DNA-Directed DNA Polymerase
- Escherichia coli
- HeLa Cells
- Humans
- Models, Genetic
- Molecular Sequence Data