Modified Taq DNA Polymerase for Allele-Specific Ultra-Sensitive Detection of Genetic Variants.

doi:10.1016/j.jmoldx.2022.08.002

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Article

Modified Taq DNA Polymerase for Allele-Specific Ultra-Sensitive Detection of Genetic Variants.

The Journal of molecular diagnostics : JMD - 1 Nov 2022

Lim Youngshin, Park Il-Hyun, Lee Huy-Ho, Baek Kyuwon, Lee Byung-Chul, Cho Ginam

Abstract excerpt

Allele-specific PCR (AS-PCR) has been used as a simple, cost-effective method for genotyping and gene mapping in research and clinical settings. AS-PCR permits the detection of single nucleotide variants and insertion or deletion variants owing to the selective extension of a perfectly matched primer (to the template DNA) over a mismatched primer. Thus, the mismatch discrimination power of the DNA polymerase is...

Topics

Humans
Taq Polymerase
Alleles
DNA
DNA Primers
Polymerase Chain Reaction

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Identifiers

PMID: 36058471
DOI: 10.1016/j.jmoldx.2022.08.002
PMCID: PMC9746316

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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