Symmetric lac operator derivatives: effects of half-operator sequence and spacing on repressor affinity.

Abstract excerpt

We have analyzed lac repressor binding in vivo and in vitro to several symmetric lac operator sequences. Two features of the operator appear to be important for repressor binding: sequence, both of the operator and of its extended regions, and the spacing of the operator halves. Host mutations that alter DNA superhelical density (topA, gyrB) did not change the relative affinity of cloned symmetric operator...

Topics

• Base Sequence
• Binding Sites
• Cloning, Molecular
• DNA, Superhelical
• Escherichia coli
• Lac Operon
• Molecular Sequence Data
• Mutation
• Repressor Proteins
• Transcription Factors