Article
Studies of the Escherichia coli Trp repressor binding to its five operators and to variant operator sequences.
European journal of biochemistry - 1 Nov 1999
Jeeves M, Evans P D, Parslow R A, Jaseja M, Hyde E I
Abstract excerpt
The Escherichia coli Trp repressor binds to promoters of very different sequence and intrinsic activity. Its mode of binding to trp operator DNA has been studied extensively yet remains highly controversial. In order to examine the selectivity of the protein for DNA, we have used electromobility shift assays (EMSAs) to study its binding to synthetic DNA containing the core sequences of each of its five operators...
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