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Article

Co-operative interactions control conjugative transfer of broad host-range plasmid RK2: full effect of minor changes in TrbA operator depends on KorB.

Molecular microbiology - 1 Aug 2003

Bingle Lewis E H, Zatyka Malgorzata, Manzoor Susan E, Thomas Christopher M

Abstract excerpt

A network of circuits, with KorB and TrbA as key regulators, controls genes for conjugative transfer of broad host range plasmid RK2. To assess the importance of the TrbA regulon, mutational analysis was applied to the TrbA operator at the trbB promoter and then to other TrbA-regulated promoters in the tra region. All identified TrbA operators are submaximal; in the case of trbBp, a G to A transition that made...

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PMID: 12890031
DOI: 10.1046/j.1365-2958.2003.03620.x

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