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Article

Point mutation at codon 12 of the Ki-ras gene in a primary breast carcinoma and the MDA-MB-134 human mammary carcinoma cell line.

Cancer letters - 30 May 1990

Prosperi M T, Dupré G, Lidereau R, Goubin G

Abstract excerpt

We found an activated Kirsten (Ki)-ras gene in the MDA-MB-134 breast carcinoma cell line by transfection of NIH3T3 cells. Oligonucleotide hybridization demonstrated that this cell line carries a single G to C point mutation at position 12 leading to a glycine-arginine substitution. However, only a fraction of the cell population seems to contain this Ki-ras mutation. Since mutations can occur in cell lines during...

Topics

Base Sequence
Breast Neoplasms
Cell Transformation, Neoplastic
Codon
DNA, Neoplasm
Genes, ras
Humans
Molecular Sequence Data
Mutation
Nucleic Acid Hybridization
Oligonucleotides

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Polymerase Chain Reaction

Tumor Cells, Cultured

Source

Identifiers

PMID: 2188722
DOI: 10.1016/0304-3835(90)90053-z

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2188722-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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