Article
A method to detect ras point mutations in small subpopulations of cells.
Analytical biochemistry - 15 May 1991
Chen J, Viola M V
Abstract excerpt
Biochemical assays for ras mutations are capable of detecting a mutant allele only if it is present in at least 5% of cells tested. Further, ras mutation assays which utilize the polymerase chain reaction (PCR) are unable to distinguish a ras mutation in a small population of cells from mutations resulting from Taq DNA polymerase base misincorporation. We used a standard restriction fragment length polymorphism...
Topics
- Base Sequence
- Codon
- DNA-Directed DNA Polymerase
- Genes, ras
- Humans
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Reproducibility of Results
- Sensitivity and Specificity