Article
Protection of adult rabbits and monkeys from lethal shigellosis by oral immunization with a thymine-requiring and temperature-sensitive mutant of Shigella flexneri Y.
Vaccine - 1 Apr 1990
Ahmed Z U, Sarker M R, Sack D A
Abstract excerpt
As an approach to the development of attenuated strains, thymine-requiring (Thy-) and temperature-sensitive (Ts-) single and double mutants of Shigella flexneri Y have been isolated by u.v. mutagenesis and selection. The mutants retained the 140 MDa plasmid, were Sereny-negative and avirulent in 'conditioned' rabbits and in monkeys. Rabbits and monkeys immunized orally with two doses of the mutants were solidly...
Topics
- Administration, Oral
- Animals
- Bacterial Vaccines
- Dysentery, Bacillary
- Electrophoresis, Polyacrylamide Gel
- Lipopolysaccharides
- Macaca mulatta
- Mutation
- Rabbits
- Shigella flexneri
- Temperature