Article
Identification of trans-dominant HIV-1 rev protein mutants by direct transfer of bacterially produced proteins into human cells.
Nucleic acids research - 25 Apr 1990
Mermer B, Felber B K, Campbell M, Pavlakis G N
Abstract excerpt
A synthetic rev gene containing substitutions which introduced unique restriction sites but did not alter the deduced amino acid sequence was used as a vehicle to construct mutations in rev. Insertion or substitution mutations within a domain of Rev resulted in proteins able to inhibit the function of Rev protein in trans. Rev function was monitored in a cell line, HLfB, which contained a rev- mutant provirus....
Topics
- Amino Acid Sequence
- Base Sequence
- Cell Line
- Cloning, Molecular
- Escherichia coli
- Gene Products, rev
- Genes, Synthetic
- Genes, Viral
- Genes, rev
- HIV-1
- Humans