Article
Analysis of trafficking of Rev and transdominant Rev proteins in living cells using green fluorescent protein fusions: transdominant Rev blocks the export of Rev from the nucleus to the cytoplasm.
Virology - 10 Nov 1995
Stauber R, Gaitanaris G A, Pavlakis G N
Abstract excerpt
Expression of gag/pol and env genes of human immunodeficiency virus requires the viral Rev protein. Mutant Rev proteins, displaying a transdominant phenotype (TDRev), were shown to inhibit Rev function. To investigate the underlying mechanism of this inhibition, the green fluorescent protein (GFP...
Topics
- Base Sequence
- Biological Transport
- Cell Line, Transformed
- Cell Nucleolus
- Cell Nucleus
- Cytoplasm
- DNA Primers
- Dactinomycin
- Gene Products, rev
- Gene Products, rex
- Green Fluorescent Proteins
- HIV Core Protein p24
- HIV-1
- HeLa Cells
- Humans
- Luminescent Proteins
- Molecular Sequence Data
- Phenotype