Article
Nuclear export of the human immunodeficiency virus type 1 nucleocytoplasmic shuttle protein Rev is mediated by its activation domain and is blocked by transdominant negative mutants.
Journal of virology - 1 Jun 1995
Szilvay A M, Brokstad K A, Kopperud R, Haukenes G, Kalland K H
Abstract excerpt
The human immunodeficiency virus type 1 nucleocytoplasmic shuttle protein Rev moves repeatedly between the cytoplasm, a perinuclear zone, the nucleoli, and nucleoplasmic speckles. In this study, we demonstrated by both indirect immunofluorescence and Western immunoblot analysis that nuclear exit...
Topics
- Animals
- Biological Transport
- Blotting, Western
- Cell Line
- Cell Nucleus
- Dactinomycin
- Fluorescent Antibody Technique
- Gene Products, rev
- Genes, Dominant
- HIV-1
- Mutation
- RNA Splicing
- RNA, Messenger
- RNA, Viral
- rev Gene Products, Human Immunodeficiency Virus