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Article

Altering enzymatic activity: recruitment of carboxypeptidase activity into an RTEM beta-lactamase/penicillin-binding protein 5 chimera.

Proceedings of the National Academy of Sciences of the United States of America - 1 Apr 1990

Chang Y H, Labgold M R, Richards J H

Abstract excerpt

The D-Ala-D-Ala carboxypeptidases/transpeptidases (penicillin-binding proteins, PBPs) share considerable structural homology with class A beta-lactamases (EC 3.5.2.6), although these beta-lactamases have no observable D-Ala-D-Ala carboxypeptidase activity. With the objective of recruiting such activity into a beta-lactamase background, we have prepared a chimeric protein by inserting a 28-amino acid segment of...

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Identifiers

PMID: 2181451
DOI: 10.1073/pnas.87.7.2823
PMCID: PMC53783

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