Article
Mutational analysis of Escherichia coli PepA, a multifunctional DNA-binding aminopeptidase.
Journal of molecular biology - 15 Sept 2000
Charlier D, Kholti A, Huysveld N, Gigot D, Maes D, Thia-Toong T L, Glansdorff N
Abstract excerpt
Escherichia coli PepA is a hexameric aminopeptidase that is also endowed with a DNA-binding activity that functions in transcription control and plasmid dimer resolution. To gain further insight into the functioning of PepA, mutants were selected on the basis of reduced repressibility of a genomic carA-lacZ fusion and studied for the various cellular processes requiring PepA, i.e. repression of the carAB operon,...
Topics
- Adenine
- Aminopeptidases
- Bacterial Proteins
- Catalysis
- Chromosomes, Bacterial
- DNA Methylation
- DNA, Bacterial
- DNA-Binding Proteins
- Dioxygenases
- Escherichia coli
- Feedback
- Gene Expression Regulation, Bacterial
- Genes, Reporter