Val-237 for Ala substitution in the TEM-2 beta-lactamase dramatically alters the catalytic efficiencies towards carbenicillin and ticarcillin.

doi:10.1111/j.1574-6968.1994.tb06788.x

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Article

Val-237 for Ala substitution in the TEM-2 beta-lactamase dramatically alters the catalytic efficiencies towards carbenicillin and ticarcillin.

FEMS microbiology letters - 15 Apr 1994

Barthélémy M, Péduzzi J, Rowlands D, Paul G, Moreau G, Labia R

Abstract excerpt

The mutant 554 of TEM-2 beta-lactamase was selected for a decrease in the resistance to carbenicillin of an Escherichia coli K12 carrier. The amino acid sequence of the mutant beta-lactamase was determined by manual Edman degradation analysis of proteolytic peptides. A single substitution Val for...

Topics

Amino Acid Sequence
Carbenicillin
Kinetics
Molecular Sequence Data
Mutation
Structure-Activity Relationship
Ticarcillin
beta-Lactamases

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