Article
Improvement of the applicability of carboxypeptidase Y in peptide synthesis by protein engineering.
Peptide research - 1 Jan 2000
Raaschou-Nielsen M, Mortensen U H, Olesen K, Breddam K
Abstract excerpt
Asn51 and Glu145 of (serine) carboxypeptidase Y function as binding sites for the C-terminal carboxylate group of peptide substrates, and Glu65 is involved in orienting these two amino acid residues. A series of mutants of carboxypeptidase Y where these three amino acid residues have been replace...
Topics
- Acylation
- Amino Acids
- Carboxypeptidases
- Esters
- Mutation
- Peptide Biosynthesis
- Protein Engineering
- Structure-Activity Relationship