Article
Relations between beta-lactamases and penicillin-binding proteins: beta-lactamase activity of penicillin-binding protein 5 from Escherichia coli.
Reviews of infectious diseases - 1 Jan 2000
Nicholas R A, Strominger J L
Abstract excerpt
The molecular mechanism of the hydrolysis of penicillin by penicillin-binding protein (PBP) 5 was investigated. Cysteine-115 had been previously implicated in the hydrolysis of the acyl-enzyme intermediate in PBP5. In studies with oligonucleotide-directed mutagenesis, the role of cysteine-115 in...
Topics
- Bacterial Proteins
- Base Sequence
- Carrier Proteins
- Cloning, Molecular
- Crystallization
- Cysteine
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Gene Expression Regulation
- Genetic Vectors
- Hexosyltransferases
- Hydrolysis
- Kinetics
- Muramoylpentapeptide Carboxypeptidase
- Mutation
- Penicillin-Binding Proteins
- Peptidyl Transferases
- Plasmids