Article
Site-directed mutagenesis of proposed active-site residues of penicillin-binding protein 5 from Escherichia coli.
The Biochemical journal - 15 Oct 1994
van der Linden M P, de Haan L, Dideberg O, Keck W
Abstract excerpt
Alignment of the amino acid sequence of penicillin-binding protein 5 (PBP5) with the sequences of other members of the family of active-site-serine penicillin-interacting enzymes predicted the residues playing a role in the catalytic mechanism of PBP5. Apart from the active-site (Ser44), Lys47, S...
Topics
- Acylation
- Amino Acid Sequence
- Bacterial Proteins
- Carboxypeptidases
- Carrier Proteins
- Catalysis
- Conserved Sequence
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Hexosyltransferases
- Kinetics
- Molecular Sequence Data
- Molecular Weight
- Muramoylpentapeptide Carboxypeptidase
- Mutagenesis, Site-Directed
- Mutation
- Penicillin-Binding Proteins
- Penicillins