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Article

Increased proteolytic resistance of ribonuclease A by protein engineering.

Protein engineering - 1 Oct 2001

Markert Y, Köditz J, Mansfeld J, Arnold U, Ulbrich-Hofmann R

Abstract excerpt

Although highly stable toward unfolding, native ribonuclease A is known to be cleaved by unspecific proteases in the flexible loop region near Ala20. With the aim to create a protease-resistant ribonuclease A, Ala20 was substituted for Pro by site-directed mutagenesis. The resulting mutant enzyme was nearly identical to the wild-type enzyme in the near-UV and far-UV circular dichroism spectra, in its activity to...

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PMID: 11739898
DOI: 10.1093/protein/14.10.791

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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