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Article

One-step random mutagenesis by error-prone rolling circle amplification.

Nucleic acids research - 26 Oct 2004

Fujii Ryota, Kitaoka Motomitsu, Hayashi Kiyoshi

Abstract excerpt

In vitro random mutagenesis is a powerful tool for altering properties of enzymes. We describe here a novel random mutagenesis method using rolling circle amplification, named error-prone RCA. This method consists of only one DNA amplification step followed by transformation of the host strain, without treatment with any restriction enzymes or DNA ligases, and results in a randomly mutated plasmid library with...

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Identifiers

PMID: 15507684
DOI: 10.1093/nar/gnh147
PMCID: PMC528823

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