Article
Simple and efficient oligonucleotide-directed mutagenesis using one primer and circular plasmid DNA template.
Gene analysis techniques - 1 Jan 2000
Marotti K R, Tomich C S
Abstract excerpt
A rapid and simple procedure for site-directed mutagenesis is described. This method uses only a single oligonucleotide primer with the double-stranded circular plasmid DNA as the template for mutagenesis. The phage T4 gene 32 product is included during primer extension in vitro to increase effic...
Topics
- Animals
- Base Sequence
- Cattle
- DNA, Circular
- DNA-Binding Proteins
- Drug Resistance, Microbial
- Escherichia coli
- Genetic Techniques
- Growth Hormone
- Kanamycin
- Molecular Sequence Data
- Mutation
- Oligonucleotides
- Plasmids
- T-Phages
- Templates, Genetic
- Tissue Plasminogen Activator
- Transformation, Genetic