Article
Inverse PCR for Site-Directed Mutagenesis.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2023
Silva Diogo, Santos Gustavo, Barroca Mário, Costa Diogo, Collins Tony
Abstract excerpt
Inverse PCR is a powerful tool for the rapid introduction of desired mutations at desired positions in a circular double-stranded DNA sequence. In this technique, custom-designed mutant primers oriented in the inverse direction are used to amplify the entire circular template with incorporation of the required mutation(s). By careful primer design, it can be used to perform such diverse modifications as the...
Topics
- Mutagenesis, Site-Directed
- Polymerase Chain Reaction
- Mutation
- Protein Engineering
- Temperature