How many EF-Tu molecules participate in aminoacyl-tRNA binding and peptide bond formation in Escherichia coli translation?

doi:10.1016/0022-2836(90)90074-V

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Article

How many EF-Tu molecules participate in aminoacyl-tRNA binding and peptide bond formation in Escherichia coli translation?

Journal of molecular biology - 20 Feb 1990

Ehrenberg M, Rojas A M, Weiser J, Kurland C G

Abstract excerpt

We have observed that two EF-Tu.GTP cycles are required to make one peptide bond during steady-state translation in an accurate and fast poly(U) translation system prepared from Escherichia coli. We have also found that there are two complexes of EF-Tu.GTP bound to one molecule of aminoacyl-tRNA...

Topics

Escherichia coli
Guanosine Triphosphate
Kinetics
Leucine-tRNA Ligase
Mutation
Peptide Elongation Factor Tu
Phenylalanine-tRNA Ligase
Poly U
Protein Binding
Protein Biosynthesis
RNA, Transfer, Amino Acyl
Ribosomes

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