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Article

Effect of Sub-Atmospheric Oxygen on the Culture of Rabbit Limbal Epithelial Cells

22 Jul 2011

Abstract excerpt

PURPOSE: To investigate the effect of sub-atmospheric oxygen tensions on the maintenance and expansion of limbal epithelial cells in vitro. MATERIALS AND METHODS: Limbal epithelial cells were isolated from rabbit corneas and cultured in 21%, 14%, 8%, and 2% oxygen in a co-culture system with Mitomycin C growth arrested 3T3 fibroblasts. The colony forming efficiency, proliferative ability, and cell cycle...

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PMID: 21780918
DOI: 10.3109/02713683.2011.556302

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