Article
The use of hyperoxia to induce chronic mild oxidative stress in RPE cells in vitro.
Molecular vision - 14 Mar 2001
Honda S, Hjelmeland L M, Handa J T
Abstract excerpt
PURPOSE: To establish a model of mild and chronic oxidative stress using hyperoxia for retinal pigment epithelial (RPE) cells in vitro. METHODS: RPE340 cells and WI38 lung fibroblasts were grown in normal oxygen (20% O2) and hyperoxia (40% O2 or 60% O2). After cell viability was examined, the levels of reactive oxygen intermediates (ROI) by flow cytometry and heme oxygenase-1 (HO-1) mRNA by northern analysis were...
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