Article
Simultaneous maintenance of human cord blood SCID-repopulating cells and expansion of committed progenitors at low O2 concentration (3%).
Stem cells (Dayton, Ohio) - 1 Jan 2004
Ivanovic Zoran, Hermitte Francis, Brunet de la Grange Philippe, Dazey Bernard, Belloc Francis, Lacombe Francis, Vezon Gérard, Praloran Vincent
Abstract excerpt
In the present work, we tested the hypothesis that liquid cultures (LCs) of cord blood CD34+ cells at an appropriate low O2 concentration could simultaneously allow colony-forming cell (CFC) expansion and nonobese diabetic/severe combined immunodeficiency mice-repopulating cell (SRC) maintenance. We first found that 3% was the minimal O2 concentration, still allowing the same rate of CFC expansion as at 20% O2....
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