Article
Effects of fibroblast origin and phenotype on the proliferative potential of limbal epithelial progenitor cells.
Experimental eye research - 1 Jan 2011
Ainscough S Louise, Linn May L, Barnard Zeke, Schwab Ivan R, Harkin Damien G
Abstract excerpt
The current study investigates potential differences in fibroblast phenotype across the anterior segment of the human eye with the aim to understanding factors that support the regenerative function of human limbal epithelial progenitor cells (LEPs) during wound healing. Separate cultures of fibroblasts were established from the cornea, limbus and sclera by growth in serum-supplemented medium. The resulting...
Topics
- Biomarkers
- Blotting, Western
- Cell Culture Techniques
- Cell Lineage
- Cell Proliferation
- Coculture Techniques
- Cornea
- Epithelial Cells
- Fibroblasts
- Flow Cytometry
- Fluorescent Antibody Technique, Indirect
- Humans
- Limbus Corneae
- Phenotype
- Sclera
- Stem Cells
- Stromal Cells
- Thy-1 Antigens