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Article

Preservation of the limbal stem cell phenotype by appropriate culture techniques.

Investigative ophthalmology & visual science - 1 Feb 2010

Meyer-Blazejewska Ewa Anna, Kruse Friedrich E, Bitterer Katrin, Meyer Christian, Hofmann-Rummelt Carmen, Wünsch Peter H, Schlötzer-Schrehardt Ursula

Abstract excerpt

PURPOSE: To evaluate the effect of several culture variables on clonal growth and differentiation of limbal stem cells ex vivo and provide an improved culture technique that supports preferential expansion and preservation of stem cells for therapeutic applications. METHODS: Corneal epithelial stem cells were isolated from human limbal specimens and clonally expanded on a 3T3 feeder layer, followed by...

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Identifiers

PMID: 19710417
DOI: 10.1167/iovs.09-4109

Genes

ABCG2
EGF
NGF

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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