Mutations that affect Tn5 insertion into pBR322: importance of local DNA supercoiling.

doi:10.1128/jb.172.10.5956-5960.1990

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Article

Mutations that affect Tn5 insertion into pBR322: importance of local DNA supercoiling.

Journal of bacteriology - 1 Oct 1990

Lodge J K, Berg D E

Abstract excerpt

The major hot spot of transposon Tn5 insertion in plasmid pBR322 (hot spot I) is in the promoter for the tetracycline resistance gene (tet). We made a series of pBR322 derivatives with mutations in and around this promoter and assayed their effects on insertion of Tn5 into hot spot I. Those mutat...

Topics

Base Sequence
DNA Transposable Elements
DNA, Superhelical
Escherichia coli
Molecular Sequence Data
Mutation
Plasmids
Restriction Mapping

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