Article
IS10 transposase mutations that specifically alter target site recognition.
The EMBO journal - 1 Feb 1992
Bender J, Kleckner N
Abstract excerpt
IS10 inserts preferentially into particular hotspots. We describe here mutations of IS10 transposase, called 'ATS' that confer Altered Target Specificity. These mutations yield a general relaxation in target specificity but do not affect other aspects of transposition. Thus, the preference for specific nucleotide sequences at the target site can be cleanly separated from other steps of the transposition reaction....
Topics
- Alleles
- Bacteriophage lambda
- Base Composition
- Base Sequence
- Conjugation, Genetic
- Crosses, Genetic
- DNA Transposable Elements
- Escherichia coli
- Molecular Sequence Data
- Mutagenesis, Insertional
- Mutagenesis, Site-Directed