Article
Vector for IS element entrapment and functional characterization based on turning on expression of distal promoterless genes.
Gene - 26 Sept 1996
Szeverényi I, Hodel A, Arber W, Olasz F
Abstract excerpt
We constructed and characterized a novel trap vector for rapid isolation of insertion sequences. The strategy used for the isolation of IS elements is based on the ability of many IS elements to turn on the expression of otherwise silent genes distal to some sites of insertion. The simple transposition of an IS element can sometimes cause the constitutive expression of promoterless antibiotic resistance genes...
Topics
- DNA Transposable Elements
- Escherichia coli
- Genetic Vectors
- Mutation